Multimodal characterization of skin wound healing in vivo using label-free multiphoton microscopy

Kyle P. Quinn; Jake D. Jones; Marcos R. Rodriguez; Michael J. Blair

doi:10.1117/12.2610133

2 March 2022 Multimodal characterization of skin wound healing in vivo using label-free multiphoton microscopy

Kyle P. Quinn, Jake D. Jones, Marcos R. Rodriguez, Michael J. Blair

Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC1197309 (2022) https://doi.org/10.1117/12.2610133
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Chronic skin wounds are a tremendous clinical problem, and our understanding of them has been limited by a lack of established tools and biomarkers sensitive to different wound features. Using label-free two-photon excited fluorescence, second harmonic generation, and coherent anti-Stokes Raman scattering microscopy, we demonstrate the ability to monitor skin wounds in vivo over 10 days. Specifically, differences in skin wound epithelial metabolism were identified among aged, diabetic, and young mice. Recent work involving automated wound segmentation through convolutional neural networks indicate an ability to provide rapid quantitative readouts of different aspects of wound structure and function.

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Citation Download Citation

Kyle P. Quinn, Jake D. Jones, Marcos R. Rodriguez, and Michael J. Blair "Multimodal characterization of skin wound healing in vivo using label-free multiphoton microscopy", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC1197309 (2 March 2022); https://doi.org/10.1117/12.2610133

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KEYWORDS

In vivo imaging

Multiphoton microscopy

Skin

Wound healing

Diagnostics

Mode conditioning cables

Harmonic generation

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