Laser Microfluorometer For Fluorescence Decay Measurements In Biology

A. Andreoni; A. Longoni; G. Bottiroli; G. Prenna

doi:10.1117/12.955338

10 April 1977 Laser Microfluorometer For Fluorescence Decay Measurements In Biology

A. Andreoni, A. Longoni, G. Bottiroli, G. Prenna

Proceedings Volume 0099, 3rd European Electro-Optics Conf; (1977) https://doi.org/10.1117/12.955338
Event: Third European Electro-Optics Conference, 1976, Geneva, Switzerland

Abstract

A laser microfluorometer is described which is particularly suitable for the measurement of fluorescence decays in microscopic biological samples. The apparatus makes use of (1) a nitrogen pumped dye laser of special design as the excitation source, (2) a microscope system particularly suitable for fluorescence microscopy and (3) an electronic signal averager. The main properties of this instrument include high temporal resolution (~0.2 ns) and high spatial resolution (~0.5μm). This instrument is now being used to study the fluorescence properties of complexes formed by acridine dyes with deoxyribonucleic acid (DNA). Preliminary results indicate that specific information about DNA base-pair sequences and DNA microscopic structure in chromosomes can be obtained.

Citation Download Citation

A. Andreoni, A. Longoni, G. Bottiroli, and G. Prenna "Laser Microfluorometer For Fluorescence Decay Measurements In Biology", Proc. SPIE 0099, 3rd European Electro-Optics Conf, (10 April 1977); https://doi.org/10.1117/12.955338

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