Computer Vision And Sampling Considerations In Tv Microscopy

H. Harms; H. M. Aus

doi:10.1117/12.934595

1 November 1982 Computer Vision And Sampling Considerations In Tv Microscopy

H. Harms, H. M. Aus

Proceedings Volume 0375, Medical Imaging and Image Interpretation; (1982) https://doi.org/10.1117/12.934595
Event: 1st International Symposium on Medical Imaging and Image Interpretation, 1982, Berlin, Germany

Abstract

One critical and often neglected factor in TV microscopy is the sampling rate necessary to mea-sure the many subcellular particles seen in the light microscope. The size of many of these cell components is in the same range as the limit of the resolving power of the light microscope. Chromatin distribution patterns in the nuclei, cytoplasm granulation, and chromosomes are only a few examples. The correct sampling of these small particles requires a sampling rate greater than the Nyquist criteria postulate for an ideal system because the sampling function and the MTF of the microscope is not precisely band limited. However, too large sampling rates lead to truncation errors as well as huge amounts of data which have to be processed. This paper shows how the sampling rate can be estimated from the aliasing error and truncation error. The required sample rate to analyse chromosomes and leukemic cells for example is in the range between 20 and 30 pixels/micron.

Citation Download Citation

H. Harms and H. M. Aus "Computer Vision And Sampling Considerations In Tv Microscopy", Proc. SPIE 0375, Medical Imaging and Image Interpretation, (1 November 1982); https://doi.org/10.1117/12.934595

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