Calcium Activation Profile In Electrically Stimulated Intact Rat Heart Cells

Hugo Geerts; Rony Nuydens; Luc Ver Donck; Roger Nuyens; Marc De Brabander; Marcel Borgers

doi:10.1117/12.945396

24 June 1988 Calcium Activation Profile In Electrically Stimulated Intact Rat Heart Cells

Hugo Geerts, Rony Nuydens, Luc Ver Donck, Roger Nuyens, Marc De Brabander, Marcel Borgers

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945396
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

Recent advances in fluorescent probe technology and image processing equipment have made available the measurement of calcium in living systems on a real-time basis. We present the use of the calcium indicator Fura-2 in intact normally stimulated rat heart cells for the spatial and dynamic measurement of the calcium excitation profile. After electric stimulation (1 Hz), the activation proceeds from the center of the myocyte toward the periphery. Within two frame times (80 ms), the whole cell is activated. The activation is slightly faster in the center of the cell than in the periphery. The mean recovery time is 200-400 ms. There is no difference along the cell's long axis. The effect of a beta-agonist and of a calcium antagonist is described.

Citation Download Citation

Hugo Geerts, Rony Nuydens, Luc Ver Donck, Roger Nuyens, Marc De Brabander, and Marcel Borgers "Calcium Activation Profile In Electrically Stimulated Intact Rat Heart Cells", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945396

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