Multifrequency Phase-Resolution For Total Luminescence Spectroscopy

Linda B McGown; David W Millican

doi:10.1117/12.945414

24 June 1988 Multifrequency Phase-Resolution For Total Luminescence Spectroscopy

Linda B McGown, David W Millican

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945414
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

Multifrequency phase-resolved fluorescence spectroscopy is used to generate a multidimensional data array in which phase-resolved fluorescence intensity is measured as a function of emission wavelength and excitation wavelength at a series of excitation modulation frequencies. The dependence of the phase-resolved fluorescence intensity on fluorescence lifetime, modulation frequency and detector phase angle setting is examined. A model two-component system of benzo(k)fluoranthene and benzo(b)fluoranthene has been used to demonsrate the ability of phase-resolved fluorescence spectroscopy to selectively enhance the spectral contribution of a component as a function of fluorescence lifetime in total luminescence spectroscopyl, and is also discussed here.

Citation Download Citation

Linda B McGown and David W Millican "Multifrequency Phase-Resolution For Total Luminescence Spectroscopy", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945414

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