Recovery Of Fluorescence Lifetimes And Underlying Distributions Of Human Blood Serum With A Fast Analog Technique

Nancy A Miller; S Gangopadhyay; Walter L Borst

doi:10.1117/12.945388

24 June 1988 Recovery Of Fluorescence Lifetimes And Underlying Distributions Of Human Blood Serum With A Fast Analog Technique

Nancy A Miller, S Gangopadhyay, Walter L Borst

Author Affiliations +

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945388
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

Two methods of analysis have been used to determine the lifetimes of human blood serum fluorescence by our fast analog technique. The first method models the serum fluorescence with up to two components, with lifetimes and pre-exponential coefficients of the multi-exponential decay determined by the Marquardt algorithm. Although this model provides a good fit, it is evident that not all components are discerned. The second method assumes an underlying distribution of lifetimes. We have applied James and Ware's series exponential method to the fluorescence decay of anthracene (discrete lifetime), a fluorescence probe [p-(N,N-dialkylamino)benzylidene]malononitrile in polymethyl methacrylate, and human serum (distribution of lifetimes). We found for our experimental technique that the percentage contributions (rather than the pre-exponential coefficients) of each lifetime to the total intensity should be set equal at the beginning of the iterations. Up to 22 lifetimes were used to fit the fluorescence data. This method was applied to a fluorescence assay to develop a calibration curve when a background signal was significant.

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Nancy A Miller, S Gangopadhyay, and Walter L Borst "Recovery Of Fluorescence Lifetimes And Underlying Distributions Of Human Blood Serum With A Fast Analog Technique", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945388

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