Resolution Of Overlapping Spectra Using Frequency-Domain Fluorometry

Beniamino F Barbieri; Fabio De Piccoli; Enrico Gratton

doi:10.1117/12.945415

24 June 1988 Resolution Of Overlapping Spectra Using Frequency-Domain Fluorometry

Beniamino F Barbieri, Fabio De Piccoli, Enrico Gratton

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945415
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

Commercial high-resolution phase and modulation fluorometers allow real time resolution of excitation and emission spectra of fluorophores with different lifetimes in a mixture. The technique can also be used for the separation of fluorescence and phosphorescence spectra of the fluorophore.

Citation Download Citation

Beniamino F Barbieri, Fabio De Piccoli, and Enrico Gratton "Resolution Of Overlapping Spectra Using Frequency-Domain Fluorometry", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945415

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