Feasibility of using RH795 dye for photoacoustic imaging of neuro-electrical activity

Nashaat Rasheed; John R. Cressman; Parag V. Chitnis

doi:10.1117/12.2253767

8 February 2017 Feasibility of using RH795 dye for photoacoustic imaging of neuro-electrical activity

Nashaat Rasheed, John R. Cressman, Parag V. Chitnis

Proceedings Volume 10051, Neural Imaging and Sensing; 100510X (2017) https://doi.org/10.1117/12.2253767
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Currently, the most researched noninvasive approach for monitoring neuro-electrical activity involves opticalfluorescence imaging, which suffers from limited imaging penetration. We propose an alternative approach, photoacoustic imaging (PAI) of biopotentials, that relies on transient absorption of light by voltage-sensitive probes and subsequent generation/detection of ultrasound. PAI-based voltage imaging approach can offer the same advantages as the fluorescence imaging in terms of sensitivity and molecular specificity, but it also can significantly extend the imaging depth. In this pilot study we are investigating the feasibility of photoacoustically visualizing biopotentials in rat pheochromocytoma (PC12) cells tagged with voltage-sensitive styrylpyridinium dye, RH795. A change in the intramembrane potential was induced in PC12 cells by adding tetraphenylborate (TPB) to the cell culture. A custommade absorption spectrophotometer was used to verify the change in optical absorption of RH795 dye as a result of TPBinduced electrical fields. Absorption spectra recorded before and after the addition of 100 μM TPB exhibited a wavelength shift of the absorption peak (approximately 510 nm to 550 nm) as well as an increase in the overall magnitude of absorption in the wavelength range of 500-1000 nm. The absorption spectral measurements indicated that RH795 is a good candidate as a voltage-sensitive dye for photoacoustically tracking changes in cell-membrane potential.

Citation Download Citation

Nashaat Rasheed, John R. Cressman, and Parag V. Chitnis "Feasibility of using RH795 dye for photoacoustic imaging of neuro-electrical activity", Proc. SPIE 10051, Neural Imaging and Sensing, 100510X (8 February 2017); https://doi.org/10.1117/12.2253767

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