Probing single processive molecular motors with high-speed optical tweezers and fluorescence microscopy

L. Gardini; F. S. Pavone; M. Capitanio

doi:10.1117/12.2256564

21 February 2017 Probing single processive molecular motors with high-speed optical tweezers and fluorescence microscopy

L. Gardini, F. S. Pavone, M. Capitanio

Author Affiliations +

Proceedings Volume 10071, Single Molecule Spectroscopy and Superresolution Imaging X; 1007111 (2017) https://doi.org/10.1117/12.2256564
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Here we present development of optical techniques for the study of single processive myosin motors based on the combination of high-speed optical tweezers force spectroscopy and single molecule fluorescence imaging. Ultrafast force-clamp spectroscopy¹ is applied to study the dependence of single chemo-mechanical steps of processive myosin motors on the applied load. On the other hand, single molecule localization through FIONA (Fluorescence Imaging with One Nanometer Accuracy)^{2, 3} is applied to in vitro motility assay to measure parameters such as the runlength, velocity and step size of single myosin V motors, labeled with Quantum Dots, under unloaded conditions.

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L. Gardini, F. S. Pavone, and M. Capitanio "Probing single processive molecular motors with high-speed optical tweezers and fluorescence microscopy", Proc. SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X, 1007111 (21 February 2017); https://doi.org/10.1117/12.2256564

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