Ring resonator for biosensing via flow-through approach (Conference Presentation)

Romeo Bernini; Immacolata Angelica Grimaldi; Gianluca Persichetti; Genni Testa

doi:10.1117/12.2253612

2 May 2017 Ring resonator for biosensing via flow-through approach (Conference Presentation)

Romeo Bernini, Immacolata Angelica Grimaldi, Gianluca Persichetti, Genni Testa

Proceedings Volume 10081, Frontiers in Biological Detection: From Nanosensors to Systems IX; 100810C (2017) https://doi.org/10.1117/12.2253612
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The realization of a simple real time biosensor, in which antibodies are immobilized onto surfaces, represents a promising application in the immunoassay development. Among the various sensing approaches, one of the most promising is based on microring resonators, offering a lot of advantages such as mass production, reduced dimensions, label-free and real time detection. The use of the evanescent field as optical transduction principle allows the development of label-free biosensors, in which the antibody is usually immobilized on the sensor surface and the binding of the antigen can be controlled and followed in real-time. The overall performances of immunosensors are strongly related to the optimization of the immobilization process and the integration between the microfluidic parts and the optical detection system. The combination of these two aspects makes the biosensing process very efficient, with a consequent reduction of the response time and improvement of the immobilization process efficiency. In this work we explore the working mechanism of a flow-through microresonator platform. A drilled hole, in the center of the ring, allows the active transport mechanism of the analyte toward the sensing surface with a consequent reduction of the response time. Moreover, we study the effects of oxygen plasma, in terms of duration times and plasma power, on immobilization efficiency of immunoglobulin G (IgG). An improvement of about 20% of the protein adsorption is ascribed to chemico-physical modification of SU-8. The measured sensor response time in flow-through configuration is about five times shorter respect to standard flow-over configuration.

Conference Presentation

Citation Download Citation

Romeo Bernini, Immacolata Angelica Grimaldi, Gianluca Persichetti, and Genni Testa "Ring resonator for biosensing via flow-through approach (Conference Presentation)", Proc. SPIE 10081, Frontiers in Biological Detection: From Nanosensors to Systems IX, 100810C (2 May 2017); https://doi.org/10.1117/12.2253612

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Biosensing

Resonators

Biosensors

Plasma

Sensors

Active remote sensing

Integrated optics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years