Silver nanoparticles as optical clearing agent enhancers to improve caries diagnostic by optical coherence tomography

Vanda S. M. Carneiro; Cláudia C. B. O. Mota; Alex F. Souza; Evair J. da Silva; Andrea F. da Silva; Marleny E. M. M. Gerbi; Anderson S. L. Gomes

doi:10.1117/12.2290671

23 February 2018 Silver nanoparticles as optical clearing agent enhancers to improve caries diagnostic by optical coherence tomography

Vanda S. M. Carneiro, Cláudia C. B. O. Mota, Alex F. Souza, Evair J. da Silva, Andrea F. da Silva, Marleny E. M. M. Gerbi, Anderson S. L. Gomes

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Proceedings Volume 10507, Colloidal Nanoparticles for Biomedical Applications XIII; 1050719 (2018) https://doi.org/10.1117/12.2290671
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

The use of silver nanoparticles as optical clearing agent (OCA) enhancers to improve caries diagnostic by optical coherence tomography (OCT) is demonstrated here. Five molars with no evident cavitation were selected. The OCAs were based on aqueous solution of silver nanoparticles (AgNP, 1.18x 10¹⁴ particles/mL, ø ≈ 10nm) and its dilution at 10% in glycerol. Teeth were placed on a platform with a micrometric screw, and after applying the OCAs, they were scanned with a Callisto SD-OCT system operating ate 930nm central wavelength. The occlusal surfaces were scanned by OCT, capturing crosssectional images with 8 mm transversal scanning, generating numerical matrices (2000x512). The OCT images had their transverse dimension preserved. AgNP-OCAs promoted image stretching due to the modification in the light optical path caused by AgNP-OCAs refractive indices close to that of the enamel. AgNP-OCAs evidenced the enamel birefringence and highlighted initial demineralization areas, that presented defined margins with higher contrast between sound and demineralized regions, with higher OCT signal intensity in those areas.

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Vanda S. M. Carneiro, Cláudia C. B. O. Mota, Alex F. Souza, Evair J. da Silva, Andrea F. da Silva, Marleny E. M. M. Gerbi, and Anderson S. L. Gomes "Silver nanoparticles as optical clearing agent enhancers to improve caries diagnostic by optical coherence tomography", Proc. SPIE 10507, Colloidal Nanoparticles for Biomedical Applications XIII, 1050719 (23 February 2018); https://doi.org/10.1117/12.2290671

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KEYWORDS

Optical coherence tomography

Dental caries

Tissues

Nanoparticles

Silver

Refractive index

Optical clearing

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