Imaging Flow Cytometer

Chi Keung Yeung; Peter Nickolls; Mel Clarke; Sim Heng Ong; David Horne

doi:10.1117/12.951894

13 June 1989 Imaging Flow Cytometer

Chi Keung Yeung, Peter Nickolls, Mel Clarke, Sim Heng Ong, David Horne

Proceedings Volume 1063, New Technologies in Cytometry; (1989) https://doi.org/10.1117/12.951894
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

An imaging flow cytometer is described in this paper. It possesses the high cell processing rate of a flow cytometer and the high image resolution of an automated microscope. It is a flow cytometer to which has been added a cell detector and velocity measuring circuit and a microscope objective lens that focuses cell illuminated by a laser onto a two-dimensional charge-coupled device (CCD) array. Optical sensing and imaging are carried out in a water filled chamber to reduce optical abberration. The time-delay and integration (TDI) technique is utilized for image acquisition. Image processing and classification is to be carried out in real time by a n-channel, metal-oxide-semicoductor (nMOS) parallel processor array. This imaging flow cytometer can acquires image at a flow rate of 0.5 m/s and in the future will be capable of analysing and sorting cells at high speed on the basis of simultaneous morphology as well as cytochemistry and immunology.

Citation Download Citation

Chi Keung Yeung, Peter Nickolls, Mel Clarke, Sim Heng Ong, and David Horne "Imaging Flow Cytometer", Proc. SPIE 1063, New Technologies in Cytometry, (13 June 1989); https://doi.org/10.1117/12.951894

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