CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 10724 > Article
Presentation
17 September 2018 QD-TSPP FRET systems for fluorescent sensing (Conference Presentation)
Yuqian Liu, Qingjiang Sun
Author Affiliations +
Proceedings Volume 10724, Physical Chemistry of Semiconductor Materials and Interfaces XVII; 107241A (2018) https://doi.org/10.1117/12.2320815
Event: SPIE Nanoscience + Engineering, 2018, San Diego, California, United States
Abstract
Quantum dots (QDs)-based fluoresecnce resonance energy transfer (FRET) processes are implemented to develop a variety of optical sensors. Herein we report the construction of dual-colored QDs-based binary FRET (BiFRET) systems and demonstrate their feasibility for ratiometric detection of Zn2+. The QD-FRET system was constructed by first coating of spacer on the QD donor, followed by loading of multiple acceptors, meso-tetra(4-sulfonatophenyl)porphine dihydrochloride (TSPP), on the spacer. The spacer thickness controlled the QD-TSPP FRET distance, determining the FRET efficiency. Silica, DNA and poly(dA) were used, respectively, to control the QD-TSPP FRET distance and demonstrated different FRET efficiencies. The biocopolymer poly(dA) had the minimal space thickness of 1.7 nm, achieving the highest QD-TSPP FRET efficency of 70%. By mixing two QD-FRET systems, a QD-BiFRET system (FRET-1 for 517QD-TSPP, FRET-2 for 560QD-TSPP) was facilely prepared, which could be used for Zn2+ detection. In the absence of Zn2+, FRET-1 is efficient while FRET-2 is inefficient. In the presence of Zn2+, the chelation of Zn2+ changed the TSPP absorption at 515 nm and 555 nm in an oposite manner. Accordingly, FRET-1 became inefficient and FRET-2 became efficient. As a result, the QD-BiFRET system realized ratiometric detection of Zn2+. The system with the poly(dA) spacer had obtained the detection limit of 1 nM, which is sixtieth part of that of the system with the silica spacer. This work is financially supported by NSFC (Grant: 21775021).
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Yuqian Liu and Qingjiang Sun "QD-TSPP FRET systems for fluorescent sensing (Conference Presentation)", Proc. SPIE 10724, Physical Chemistry of Semiconductor Materials and Interfaces XVII, 107241A (17 September 2018); https://doi.org/10.1117/12.2320815
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Fluorescence resonance energy transfer
Sensing systems
Silica
Absorption
Binary data
Coating
Optical sensors
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top