Measuring sub-nanometre thickness changes during phase transitions of supported lipid bilayers with quantitative differential interference contrast microscopy

David Regan; Joseph Williams; Francesco Masia; Paola Borri; Wolfgang Langbein

doi:10.1117/12.2510461

4 March 2019 Measuring sub-nanometre thickness changes during phase transitions of supported lipid bilayers with quantitative differential interference contrast microscopy

David Regan, Joseph Williams, Francesco Masia, Paola Borri, Wolfgang Langbein

Author Affiliations +

Proceedings Volume 10887, Quantitative Phase Imaging V; 1088719 (2019) https://doi.org/10.1117/12.2510461
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Quantitative differential interference contrast (qDIC) microscopy is applied to the study of the main phase transition of dipentadecanoylphosphatidylcholine (DC₁₅PC) supported lipid bilayers. We measure thickness changes of about 1nm occurring in the bilayer with sub-nanometre resolution and show how the presence of fluorescently labelled lipids, even at small concentrations, can broaden the phase transition.

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David Regan, Joseph Williams, Francesco Masia, Paola Borri, and Wolfgang Langbein "Measuring sub-nanometre thickness changes during phase transitions of supported lipid bilayers with quantitative differential interference contrast microscopy", Proc. SPIE 10887, Quantitative Phase Imaging V, 1088719 (4 March 2019); https://doi.org/10.1117/12.2510461

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