CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 11243 > Article
Presentation
9 March 2020 A simple, compact and robust phase and fluorescence microscope for cell cycle study (Conference Presentation)
Ondrej Mandula, Cedric Allier, Dainel Fiole, Jean-Philippe Kleman, Francoise Lacroix, Lionel Hervé, Sophie Morales
Author Affiliations +
Proceedings Volume 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII; 112430P (2020) https://doi.org/10.1117/12.2543764
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
We designed a simple, compact and robust microscope for phase and fluorescent imaging. No mechanical movement of neither sample nor objective or any other part of the system is needed to change between the phase-contrast and fluorescence modality. We can observe a thousand cells in parallel in a single field of view with resolution down to 2 µm. We demonstrate the system on a FUCCI marked HeLa cell culture observed over several days directly in the standard incubator. We compare the obtained statistics to the flow cytometer data and show that we can produce a statistically relevant time-resolved measurement
Conference Presentation
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Ondrej Mandula, Cedric Allier, Dainel Fiole, Jean-Philippe Kleman, Francoise Lacroix, Lionel Hervé, and Sophie Morales "A simple, compact and robust phase and fluorescence microscope for cell cycle study (Conference Presentation)", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430P (9 March 2020); https://doi.org/10.1117/12.2543764
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Luminescence
Microscopes
Image segmentation
Chromatic aberrations
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top