Optical imaging methods for label free detection of microplastics in cells, tissues and environmental organisms (Conference Presentation)

Alvaro Barroso Pena; Steffi Ketelhut; Matthias Godejohann; Björn Kemper; Jürgen Schnekenburger

doi:10.1117/12.2546098

9 March 2020 Optical imaging methods for label free detection of microplastics in cells, tissues and environmental organisms (Conference Presentation)

Alvaro Barroso Pena, Steffi Ketelhut, Matthias Godejohann, Björn Kemper, Jürgen Schnekenburger

Author Affiliations +

Proceedings Volume 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII; 1124315 (2020) https://doi.org/10.1117/12.2546098
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Microplastic particles are a major environmental pollution problem. Challenges in microplastics detection are the broad size range, chemical heterogeneity and low concentrations in liquids. Label free optical imaging methods for the detection and particle type identification were tested and compared. Quantitative Phase Imaging and holographic tomography were used to study cell response and cell uptake of microplastic particles. Optical Coherence Tomography could analyze larger organisms as Daphnia magna for microplastics uptake. Imaging methods combining particle localization with a chemical identification as RAMAN and mid IR spectroscopy could clearly identify different particle types and even detect specific microplastic chemistries inside cells.

Conference Presentation

Citation Download Citation

Alvaro Barroso Pena, Steffi Ketelhut, Matthias Godejohann, Björn Kemper, and Jürgen Schnekenburger "Optical imaging methods for label free detection of microplastics in cells, tissues and environmental organisms (Conference Presentation)", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 1124315 (9 March 2020); https://doi.org/10.1117/12.2546098

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