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9 March 2020 Multicolor spatial cumulant analysis to measure dopamine receptor dynamics on the plasma membrane (Conference Presentation)
Daniel J. Foust, Allesandro Ustione, David Piston
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Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 1124406 (2020) https://doi.org/10.1117/12.2543117
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
Two-photon excitation facilitates fluorescence fluctuation spectroscopy experiments by providing a well-defined observation volume that is identical across multiple emitting species. We have developed a multi-color approach, spatial cumulant analysis (SpCA), which provides images of molecular stoichiometries. We have used SpCA to determine the dynamics of dopamine receptors (DR) on the insulin secreting β-cells. There are two inhibitory DRs in the β-cell, D2 and D3, which differentially impact cAMP production, Ca2+ influx, and K+ currents. We show how multicolor spatial cumulant analysis of labeled proteins of interest can accurately measure the stoichiometry of receptors, G-proteins, and downstream signaling targets.
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Daniel J. Foust, Allesandro Ustione, and David Piston "Multicolor spatial cumulant analysis to measure dopamine receptor dynamics on the plasma membrane (Conference Presentation)", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124406 (9 March 2020); https://doi.org/10.1117/12.2543117
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KEYWORDS
Receptors
Plasma
Molecules
Diffusion
Fluorescence spectroscopy
Laser development
Luminescence
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