Multiplexed 3-photon microscopy for functional connectomics of mammalian brains

Kevin T. Takasaki; Dmitri Tsyboulski; Jack Waters

doi:10.1117/12.2543232

14 February 2020 Multiplexed 3-photon microscopy for functional connectomics of mammalian brains

Kevin T. Takasaki, Dmitri Tsyboulski, Jack Waters

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 112441F (2020) https://doi.org/10.1117/12.2543232
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

3-photon excitation enables in vivo fluorescence microscopy deep in densely labeled and highly scattering samples, while maintaining high resolution and contrast. We designed and characterized a dual-plane 3-photon microscope with temporal multiplexing and remote focusing, and performed simultaneous in vivo calcium imaging of two planes deep in the cortex of a transgenic mouse expressing GCaMP6s in nearly all excitatory neurons.

Conference Presentation

Citation Download Citation

Kevin T. Takasaki, Dmitri Tsyboulski, and Jack Waters "Multiplexed 3-photon microscopy for functional connectomics of mammalian brains", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441F (14 February 2020); https://doi.org/10.1117/12.2543232

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