Interferometric Scattering Microscopy (iSCAT): seeing and tracking nanoparticles (Conference Presentation)

Vahid Sandoghdar

doi:10.1117/12.2552983

11 March 2020 Interferometric Scattering Microscopy (iSCAT): seeing and tracking nanoparticles (Conference Presentation)

Vahid Sandoghdar

Author Affiliations +

Proceedings Volume 11249, Quantitative Phase Imaging VI; 112490G (2020) https://doi.org/10.1117/12.2552983
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Fluorescence microscopy has been the workhorse for optical microscopy but this approach confronts several fundamental limits. We show that elastic scattering, which is the basis for the most ubiquitous optical contrast mechanism, provides a very effective avenue for sensitive detection and imaging of nanoparticles and molecules at very high spatiotemporal resolution. We present interferometric scattering (iSCAT) microscopy as the method of choice, explain its theoretical foundation, elaborate on its experimental nuances, and discuss its promise and challenges in the context of applications in detection and tracking of lipids, proteins, viruses and other nanoparticles.

Conference Presentation

Citation Download Citation

Vahid Sandoghdar "Interferometric Scattering Microscopy (iSCAT): seeing and tracking nanoparticles (Conference Presentation)", Proc. SPIE 11249, Quantitative Phase Imaging VI, 112490G (11 March 2020); https://doi.org/10.1117/12.2552983

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