Light-sheet fluorescence microscopy with metalens

Cheng Hung Chu; Yuan Luo; Ming Lun Tseng; Sunil Vyas; Hsin Yu Kuo; Din Ping Tsai; Takuo Tanaka

doi:10.1117/12.2570535

20 August 2020 Light-sheet fluorescence microscopy with metalens

Cheng Hung Chu, Yuan Luo, Ming Lun Tseng, Sunil Vyas, Hsin Yu Kuo, Din Ping Tsai, Takuo Tanaka

Proceedings Volume 11500, ODS 2020: Industrial Optical Devices and Systems; 1150004 (2020) https://doi.org/10.1117/12.2570535
Event: SPIE Optical Engineering + Applications, 2020, Online Only

Abstract

We propose a Light-sheet fluorescent microscopy (LSFM) system with a flat metalens, which is used to replace the bulky illuminating components. The metalens is a diffractive optics elements (DOEs) composed of various gallium nitride (GaN) nanopillar for light-sheet generation, and it can be readily integrated in existing LSFM systems. In contrast to the traditional LSFM system, the metalens-based system is more compact and flexible, and its sectioning capability is comparable to the conventional illumination arm in LSFMs. The live fluorescent-labeled transgenic Caenorhabditis elegans (C. elegans) are exploited to verify the performance of this system. The intensity profile of oocytes in C. elegans with cellular resolution level was obtained.

Conference Presentation

Citation Download Citation

Cheng Hung Chu, Yuan Luo, Ming Lun Tseng, Sunil Vyas, Hsin Yu Kuo, Din Ping Tsai, and Takuo Tanaka "Light-sheet fluorescence microscopy with metalens", Proc. SPIE 11500, ODS 2020: Industrial Optical Devices and Systems, 1150004 (20 August 2020); https://doi.org/10.1117/12.2570535

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