Multimodal two-photon microscopy with electrical tunable lens

Noda Takumi; Xiangyu Quan; Sudheesh Rajput; Osamu Matoba; Yasuhiro Awatsuji

doi:10.1117/12.2573299

15 June 2020 Multimodal two-photon microscopy with electrical tunable lens

Noda Takumi, Xiangyu Quan, Sudheesh Rajput, Osamu Matoba, Yasuhiro Awatsuji

Proceedings Volume 11521, Biomedical Imaging and Sensing Conference 2020; 115210K (2020) https://doi.org/10.1117/12.2573299
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

In this research, we have proposed a multimodal fast fluorescent imaging by an electrical tunable lens, and quantitative phase imaging by digital holography. In conventional confocal microscopy, sectioning in z direction is realized either by moving objective lens or sample stage. In order to speed up the imaging, and utilize in the specific applications such as light stimulation system in optogenetics, the sectioning is realized by changing the focal power of an electrical tunable lens. The preliminary experiment using fluorescence beads and its relation between focal power and depth change is shown.

Citation Download Citation

Noda Takumi, Xiangyu Quan, Sudheesh Rajput, Osamu Matoba, and Yasuhiro Awatsuji "Multimodal two-photon microscopy with electrical tunable lens", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 115210K (15 June 2020); https://doi.org/10.1117/12.2573299

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