Single molecule light field microscopy

Ruth Sims; Sohaib Abdul Rehman; Martin O Lenz; Sarah I Benaissa; Ezra Bruggeman; Adam Clark; Edward W Sanders; Aleks Ponjavic; Leila Muresan; Steven F Lee; Kevin O'Holleran

doi:10.1117/12.2584562

6 October 2020 Single molecule light field microscopy

Ruth Sims, Sohaib Abdul Rehman, Martin O Lenz, Sarah I Benaissa, Ezra Bruggeman, Adam Clark, Edward W Sanders, Aleks Ponjavic, Leila Muresan, Steven F Lee, Kevin O'Holleran

Author Affiliations +

Proceedings Volume 11575, Biophotonics and Biomedical Microscopy; 1157506 (2020) https://doi.org/10.1117/12.2584562
Event: SPIE Photonex and Vacuum Expo, 2020, Online Only

Abstract

We have recently developed single molecule light field microscopy (SMLFM), a new approach to 3D single molecule localisation that is capable of up to 20 nm lateral and axial precision across a 6 micrometre depth of field. SMLFM can be readily implemented by installing a refractive microlens array into the conjugate back focal plane of a widefield single molecule localisation microscope. We have benchmarked the performance of SMLFM and showcased its capabilities by imaging fluorescently labeled membranes of fixed eukaryotic cells below the diffraction limit. In this presentation I will describe the concept of light field imaging as it is applied to single molecule localisation, its advantages, limitations and future possibilities.

Conference Presentation

Citation Download Citation

Ruth Sims, Sohaib Abdul Rehman, Martin O Lenz, Sarah I Benaissa, Ezra Bruggeman, Adam Clark, Edward W Sanders, Aleks Ponjavic, Leila Muresan, Steven F Lee, and Kevin O'Holleran "Single molecule light field microscopy", Proc. SPIE 11575, Biophotonics and Biomedical Microscopy, 1157506 (6 October 2020); https://doi.org/10.1117/12.2584562

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