Towards Fast Quantitative Wide-Field Lifetime Imaging in 2D and 3D

Xavier Intes

doi:10.1117/12.2593177

5 March 2021 Towards Fast Quantitative Wide-Field Lifetime Imaging in 2D and 3D

Xavier Intes

Proceedings Volume 11625, Molecular-Guided Surgery: Molecules, Devices, and Applications VII; 1162508 (2021) https://doi.org/10.1117/12.2593177
Event: SPIE BiOS, 2021, Online Only

Abstract

Fluorescence lifetime imaging (FLI) has become an increasingly popular method in molecular imaging as it provides unique insights into the biological processes. However, despite its popularity and profound impact, FLI is not a direct imaging modality and datasets need to be postprocessed to quantify fluorescence lifetime or lifetime-based parameters. Such technical implementations can be complex, computationally expensive, require high level of expertise as well as user inputs. Herein, we will report on the development and validation of DL models as fast and user-friendly image formation tools for FLI, including outputting the quantitative lifetime image from raw FLI measurements without iterative solvers and user input, performing FLI topography corrected by the tissue optical properties and performing end-to-end 3D optical reconstructions.

Conference Presentation

Citation Download Citation

Xavier Intes "Towards Fast Quantitative Wide-Field Lifetime Imaging in 2D and 3D", Proc. SPIE 11625, Molecular-Guided Surgery: Molecules, Devices, and Applications VII, 1162508 (5 March 2021); https://doi.org/10.1117/12.2593177

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available