Investigation of nuclear structural variations under chronic stress conditions in Allium cepa using LSFM

Rinsa S. R.; Bejoy Manoj; Mayanglambam Suheshkumar Singh

doi:10.1117/12.2583163

5 March 2021 Investigation of nuclear structural variations under chronic stress conditions in Allium cepa using LSFM

Rinsa S. R., Bejoy Manoj, Mayanglambam Suheshkumar Singh

Proceedings Volume 11647, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX; 116470B (2021) https://doi.org/10.1117/12.2583163
Event: SPIE BiOS, 2021, Online Only

Abstract

Being the largest organelle in a cell, the nucleus houses the genetic code of an organism and primarily serves as the cell’s information center. Proper implementation and consolidation of physiological stress are intrinsic for proper growth and development. Studies reveal that in animals, under chronic stress, the nuclear size shows morphological plasticity. This paper proposes the chronic stress study on plant specimens, namely, Allium cepa root, upon induction on Rhodamine B fluorophore, using our in-house developed dual arm multi-level magnification light sheet microscopy (DMx-LSFM) system. Employing our home-built microscope, we can analyze the difference in girth of the nuclear membrane under different magnification levels simultaneously, which helps to inspect the minute details and structural changes occurring in the organelle. The study was then further extended to investigate the role of exposure time and concentration of the stress agent on the sample.

Conference Presentation

Citation Download Citation

Rinsa S. R., Bejoy Manoj, and Mayanglambam Suheshkumar Singh "Investigation of nuclear structural variations under chronic stress conditions in Allium cepa using LSFM", Proc. SPIE 11647, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX, 116470B (5 March 2021); https://doi.org/10.1117/12.2583163

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available