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Standard immunofluorescence (IF) staining is labor-intensive, time-consuming and suffers from inflexibility and poor multiplicity. To overcome these limitations, we proposed a deep learning (DL) approach for virtual IF staining with high multiplicity and specificity from label-free reflectance microscopy. Our results show that DL-enabled label-free IF microscopy can predict characteristic subcellular features during different cell cycles and reveal cellular phenotypes with high accuracy.
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Shiyi Cheng, Sipei Fu, Yumi Mun Kim, Ji Yi, Lei Tian, "Multiplexed virtual fluorescence labeling from multi-contrast microscopy by deep learning," Proc. SPIE 11654, High-Speed Biomedical Imaging and Spectroscopy VI, 1165414 (5 March 2021); https://doi.org/10.1117/12.2578439