Flexing of DNA on the nucleosome core particle

Elizabeth A. Winzeler; Enoch W. Small

doi:10.1117/12.17775

1 May 1990 Flexing of DNA on the nucleosome core particle

Elizabeth A. Winzeler, Enoch W. Small

Proceedings Volume 1204, Time-Resolved Laser Spectroscopy in Biochemistry II; (1990) https://doi.org/10.1117/12.17775
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

The fluorescence anisotropy of ethidium bromide intercalated into the 146 base pairs of DNA on the nucleosoine decays due to a combination of rotational diffusion and torsional flexing motions of the DNA. We characterize the Internal flexing motions el the DNA by fitting the anisotropy to a mathematical model proposed by Schurr through the variation of two parameters: a torsional coeffident and a frictional coefficient. Using data collected 5° C and 20° C in water, and at 5° C in D₂O, and assuming a model of 146 base pairs clamped at both ends, we have in all cases obtained frictional and torsional coefficients that are higher than the values reported by others.

Citation Download Citation

Elizabeth A. Winzeler and Enoch W. Small "Flexing of DNA on the nucleosome core particle", Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); https://doi.org/10.1117/12.17775

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