Measuring the refractive index (RI) and topography of samples can be realized by decoupling the obtained quantitative phase image. However, flattening the sample, changing the surrounding medium, or illuminating sample that immerse in the highly dispersive medium with different wavelength lights encounter some problems in practice. With the help of Cauchy’s dispersion formula, RI and topography can be independently calculated from a three-wavelength-based quantitative phase imaging. Neither special highly dispersive medium nor additional assistant technology is required. After characterization of the method, the capabilities for RI measurement and substance identification are demonstrated.
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