Dynamic light scattering imaging for cell detection of HER-2 positive and triple negative breast cancer

Ting Liu; Xiangyuan Ma; Shanhang Luo; Wencong Zhang; Zequan Lin; Meiai Lin

doi:10.1117/12.2644061

19 December 2022 Dynamic light scattering imaging for cell detection of HER-2 positive and triple negative breast cancer

Ting Liu, Xiangyuan Ma, Shanhang Luo, Wencong Zhang, Zequan Lin, Meiai Lin

Author Affiliations +

Proceedings Volume 12320, Optics in Health Care and Biomedical Optics XII; 123201G (2022) https://doi.org/10.1117/12.2644061
Event: SPIE/COS Photonics Asia, 2022, Online Only

Conference Poster

Abstract

In this manuscript, we aim to develop the dynamic light scattering imaging (DLSI) method to investigate the difference between triple negative breast cancer (TNBC) and human epidermal growth factor receptor-2 (HER-2) positive breast cancer. The experimental device with the capability to obtain time-series light scattering images was well designed and built. The breast cancer cell lines of MDA-MB-231 and HER-2 enriched SKBR3 were prepared and used for experiments. The autocorrelation functions of the light intensity fluctuation were calculated to characterize both types of breast cancer cells. The dynamic light scattering images were further analyzed to establish a DLSI-based approach for automatic classification of the two types of cells. The results show that the proposed DLSI-based model achieved better classification performance compared to the conventional static light scattering-based model.

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Ting Liu, Xiangyuan Ma, Shanhang Luo, Wencong Zhang, Zequan Lin, and Meiai Lin "Dynamic light scattering imaging for cell detection of HER-2 positive and triple negative breast cancer", Proc. SPIE 12320, Optics in Health Care and Biomedical Optics XII, 123201G (19 December 2022); https://doi.org/10.1117/12.2644061

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