Handheld fluorescence test strip reader for rapid on-site biochemical detection

Huachuan Huang; Yu-Nan Lu; Yanke Shan; Fei Liu; Shouyu Wang

doi:10.1117/12.2638645

19 December 2022 Handheld fluorescence test strip reader for rapid on-site biochemical detection

Huachuan Huang, Yu-Nan Lu, Yanke Shan, Fei Liu, Shouyu Wang

Proceedings Volume 12320, Optics in Health Care and Biomedical Optics XII; 123201N (2022) https://doi.org/10.1117/12.2638645
Event: SPIE/COS Photonics Asia, 2022, Online Only

Conference Poster

Abstract

Test strips have been widely used in various rapid on-site biochemical sensing due to its simple and rapid detections. Unfortunately, many test strips only support qualitative sensing mainly because their detecting results are often determined by visual inspection. In order to solve the problem, we design a handheld fluorescence test strip reader, which can collect fluorescence emission from test strip thus quantify target under detection. The proposed handheld fluorescence test strip reader composes of rather compact optical system including a light emitting diode (LED) for excitation, an optical bandpass filter for fluorescence signal filtering and a smartphone for fluorescence signal collection and data analysis. All the optical elements are integrated using 3-D printed structures and coupled with the smartphone to support on-site detection. In addition, a smartphone application is also designed to automatically quantify fluorescence emission intensity, and it definitely can support quantitative target detection. Proved by a series of applications especially on rapid on-site detections, the handheld fluorescence test strip reader can detect target automatically, rapidly and accurately. Therefore, the proposed handheld fluorescence test strip reader is a potential tool for rapid on-site biochemical detection in various applications including agricultural, biomedical, and environmental fields.

Citation Download Citation

Huachuan Huang, Yu-Nan Lu, Yanke Shan, Fei Liu, and Shouyu Wang "Handheld fluorescence test strip reader for rapid on-site biochemical detection", Proc. SPIE 12320, Optics in Health Care and Biomedical Optics XII, 123201N (19 December 2022); https://doi.org/10.1117/12.2638645

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