Development of reference light source for micro-plate chemiluminescence analyzers

Yang Xu; Tingting Ren; Lingjian Zeng; Han Luo; Bowen Deng

doi:10.1117/12.2648358

14 October 2022 Development of reference light source for micro-plate chemiluminescence analyzers

Yang Xu, Tingting Ren, Lingjian Zeng, Han Luo, Bowen Deng

Proceedings Volume 12343, 2nd International Conference on Laser, Optics and Optoelectronic Technology (LOPET 2022); 123430K (2022) https://doi.org/10.1117/12.2648358
Event: 2nd International Conference on Laser, Optics and Optoelectronic Technology (LOPET 2022), 2022, Qingdao, China

Abstract

This article presents a novel design of reference light source and physical calibration method for optical performance evaluation of micro-plate chemiluminescence analyzers in order to substitute for traditional biological and biochemical methods. The reference light source is designed according to the working principle, and technical characteristics such as repeatability, stability, interference among wells, differences among channels and linearity of micro-plate chemiluminescence analyzers. The calibration results of 4 typical types of micro-plate chemiluminescence analyzers show that the reference light source and physical calibration method presented in this article can well simulate different chemiluminescence reagents and meet the requirements of luminous intensity calibration for chemiluminescence analyzers with the advantages of simple structure, good applicability, low cost and accurate and reliable measurement results.

Citation Download Citation

Yang Xu, Tingting Ren, Lingjian Zeng, Han Luo, and Bowen Deng "Development of reference light source for micro-plate chemiluminescence analyzers", Proc. SPIE 12343, 2nd International Conference on Laser, Optics and Optoelectronic Technology (LOPET 2022), 123430K (14 October 2022); https://doi.org/10.1117/12.2648358

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