Photonic-chip based multimodal optical imaging of formalin-fixed paraffin-embedded tissue sections (Conference Presentation)

Vishesh Kumar Dubey; Azeem Ahmad; Luis E Villegas; Hong Mao; Mona Nystad; Ganesh Acharya; Balpreet Singh Ahluwalia

doi:10.1117/12.2649860

6 March 2023 Photonic-chip based multimodal optical imaging of formalin-fixed paraffin-embedded tissue sections (Conference Presentation)

Vishesh Kumar Dubey, Azeem Ahmad, Luis E Villegas, Hong Mao, Mona Nystad, Ganesh Acharya, Balpreet Singh Ahluwalia

Author Affiliations +

Proceedings Volume 12371, Multimodal Biomedical Imaging XVIII; 1237103 (2023) https://doi.org/10.1117/12.2649860
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Histology is a well-known examination technique to study the biological cell and tissue structures. For histological assessment, imaging throughput, contrast, resolution, and quantification of morphology are crucial parameters. Although, there are techniques available which can scan the whole slide, but they lack specificity and quantification. In present study, we introduce a photonic chip based platform for multimodal imaging of FFPE tissue sections. Here, the photonic chip platform was integrated with Linnik type QPM module, which enables high contrast TIRF imaging and optical thickness of the specimen over scalable FOV. The proposed system has been used as a high throughput microscopy platform to study the functional and morphological features of FFPE human placenta tissue sections. The investigation of the tissue sections facilitates the identification and diagnosis of the various diseases, which can provide direction for treatment and can assist the prognosis of clinical outcome.

Conference Presentation

Citation Download Citation

Vishesh Kumar Dubey, Azeem Ahmad, Luis E Villegas, Hong Mao, Mona Nystad, Ganesh Acharya, and Balpreet Singh Ahluwalia "Photonic-chip based multimodal optical imaging of formalin-fixed paraffin-embedded tissue sections (Conference Presentation)", Proc. SPIE 12371, Multimodal Biomedical Imaging XVIII, 1237103 (6 March 2023); https://doi.org/10.1117/12.2649860

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