Image processing as basis for chemometrics in photothermal atomic force microscopy infrared imaging

Georg Ramer; A. Catarina V. D. dos Santos; Yide Zhang; Ufuk Yilmaz; Bernhard Lendl

doi:10.1117/12.2651424

17 March 2023 Image processing as basis for chemometrics in photothermal atomic force microscopy infrared imaging

Georg Ramer, A. Catarina V. D. dos Santos, Yide Zhang, Ufuk Yilmaz, Bernhard Lendl

Proceedings Volume 12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; 1239209 (2023) https://doi.org/10.1117/12.2651424
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Mid-IR imaging combined with machine learning is a powerful combination for non-destructive, label free chemical imaging. Key applications include computational staining and tissue classification. These applications are enabled by information rich mid-IR hyperspectral images and reliable ground truth data. As novel, nano-scale spatial resolution mid-IR spectroscopy techniques are finding broader use we realize that ground truth datasets will be needed at the nano-scale as well. Here, we propose image fusion and registration of nano-scale images as a generic approach for establishing such datasets. We demonstrate the viability of this approach for imaging the sub-cellular distribution of proteins and specific enzymes. Furthermore, we demonstrate that image registration of AFM-IR spectral data is a key step in processing AFM-IR chemical imaging data in general.

Conference Presentation

Citation Download Citation

Georg Ramer, A. Catarina V. D. dos Santos, Yide Zhang, Ufuk Yilmaz, and Bernhard Lendl "Image processing as basis for chemometrics in photothermal atomic force microscopy infrared imaging", Proc. SPIE 12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, 1239209 (17 March 2023); https://doi.org/10.1117/12.2651424

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available