Microlens flourescene imaging system for cell detection and panoramic image reconstruction algorithm

GuangLin Zhou; NingMei Yu; YaoYao Ge; Kou Wang; Jia Hao Tang; MingHui Xie

doi:10.1117/12.2668502

20 April 2023 Microlens flourescene imaging system for cell detection and panoramic image reconstruction algorithm

GuangLin Zhou, NingMei Yu, YaoYao Ge, Kou Wang, Jia Hao Tang, MingHui Xie

Proceedings Volume 12602, International Conference on Electronic Information Engineering and Computer Science (EIECS 2022); 126022Y (2023) https://doi.org/10.1117/12.2668502
Event: International Conference on Electronic Information Engineering and Computer Science (EIECS 2022), 2022, Changchun, China

Abstract

A microlens fluorescence imaging system and a panoramic image reconstruction algorithm for biological cell fluorescence detection are presented in this paper. Biological cell samples are labeled with fluorescent dyes and made into sections in a short time. The imaging system in this paper can detect the fluorescence of the samples. By modeling the optical path of the microlens imaging system and the two-dimensional mobile scanning imaging mode, the better fluorescence detection effect is obtained with the panoramic image reconstruction algorithm. The experimental results show that the microlens fluorescence imaging system can successfully collect the fluorescence information, and can reach the resolution of 2.68μm in the image sensor frame area. At the same time, the field of view area of the imaging system can reach at least 9 times or more of the sensor frame area after the reconstruction algorithm, which realizes the rapid and convenient cell fluorescence detection with

Citation Download Citation

GuangLin Zhou, NingMei Yu, YaoYao Ge, Kou Wang, Jia Hao Tang, and MingHui Xie "Microlens flourescene imaging system for cell detection and panoramic image reconstruction algorithm", Proc. SPIE 12602, International Conference on Electronic Information Engineering and Computer Science (EIECS 2022), 126022Y (20 April 2023); https://doi.org/10.1117/12.2668502

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