Difference imaging of red fluorescent proteins using pump-probe excitation

Farzin Ghane Golmohamadi; Hoang Trong Phan; Amna Mehmood; Franz-Josef Schmitt; Jan Laufer

doi:10.1117/12.2675672

11 August 2023 Difference imaging of red fluorescent proteins using pump-probe excitation

Farzin Ghane Golmohamadi, Hoang Trong Phan, Amna Mehmood, Franz-Josef Schmitt, Jan Laufer

Proceedings Volume 12631, Opto-Acoustic Methods and Applications in Biophotonics VI; 126310J (2023) https://doi.org/10.1117/12.2675672
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

Photoacoustic (PA) imaging using pump-probe excitation has been shown to detect fluorophores with high specificity. By varying the delay between the two excitation pulses and their wavelengths, the PA signal amplitude can be modulated, yielding three different and fluorophore-specific contrast mechanisms. In this study, PA images were obtained in tissue phantoms containing purified protein solutions using a ring array tomograph and a dual-OPO laser system. The results show that pump-probe excitation can detect multiple fluorescent reporters simultaneously. The method has the potential to recover biophysical parameters, such as pH and ion concentrations.

Citation Download Citation

Farzin Ghane Golmohamadi, Hoang Trong Phan, Amna Mehmood, Franz-Josef Schmitt, and Jan Laufer "Difference imaging of red fluorescent proteins using pump-probe excitation", Proc. SPIE 12631, Opto-Acoustic Methods and Applications in Biophotonics VI, 126310J (11 August 2023); https://doi.org/10.1117/12.2675672

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