Single-pixel microscopy using optical deflectors

Yingchao Li; Jaco Botha; Jadze P. C. Narag; Carl Emil S. Kovsted; Lasse P. Kristiansen; Jonas N. Pedersen; Niels B. Larsen; Emil B. Kromann

doi:10.1117/12.2685115

29 November 2023 Single-pixel microscopy using optical deflectors

Yingchao Li, Jaco Botha, Jadze P. C. Narag, Carl Emil S. Kovsted, Lasse P. Kristiansen, Jonas N. Pedersen, Niels B. Larsen, Emil B. Kromann

Author Affiliations +

Proceedings Volume 12770, Optics in Health Care and Biomedical Optics XIII; 127701H (2023) https://doi.org/10.1117/12.2685115
Event: SPIE/COS Photonics Asia, 2023, Beijing, China

Conference Poster

Abstract

We present our new microscope prototype, which uses the single-pixel imaging method to image fluorescent samples. Specifically, our microscope generates sinusoidal patterns of excitation light by interfering two laser beams ‘steered’ individually by optical deflectors. We imprint a series of such sinusoidal patterns onto the sample and use a single-pixel detector to acquire a signal-readout for each pattern. Ultimately, this allows us to ‘build’ an image of the sample. Our application of optical deflectors opens the possibility to implement a microscope that provides sharp images fast while optimizing the fluorescence duty cycle, making it suitable for long-term observation of organ-on-chip systems.

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Yingchao Li, Jaco Botha, Jadze P. C. Narag, Carl Emil S. Kovsted, Lasse P. Kristiansen, Jonas N. Pedersen, Niels B. Larsen, and Emil B. Kromann "Single-pixel microscopy using optical deflectors", Proc. SPIE 12770, Optics in Health Care and Biomedical Optics XIII, 127701H (29 November 2023); https://doi.org/10.1117/12.2685115

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