Paper
8 January 2024 The application of CRISPR/Cas9 technology in plant gene editing
Qiming Cheng
Author Affiliations +
Proceedings Volume 12924, Third International Conference on Biological Engineering and Medical Science (ICBioMed2023); 129241C (2024) https://doi.org/10.1117/12.3012857
Event: 3rd International Conference on Biological Engineering and Medical Science (ICBioMed2023), 2023, ONLINE, United Kingdom
Abstract
Due to its remarkable effectiveness, Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9 is also highlighted in gene editing with the widespread use of the technique. In order to explore a gene's low temperature tolerance or other consequences on the plant, this paper explains the research being done using the CRISPR/CAS9 system on plant genes. The article also discusses the benefits of straightforward operation, extensive target coverage, and simultaneous gene editing as well as the drawbacks of off-target consequences and the stringent laws and restrictions that apply in each nation when employing CRISPR/Cas9 for plant editing. Specifically introducing gene editing methods in plant applications can show the unique advantages of technology in gene editing. In this review article, the steps of CRISPR/Cas9 are discussed, and the problems that can be solved in plants hope can provide reference and advice for plant studies that need to be bred or growing going forward.
(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.
Qiming Cheng "The application of CRISPR/Cas9 technology in plant gene editing", Proc. SPIE 12924, Third International Conference on Biological Engineering and Medical Science (ICBioMed2023), 129241C (8 January 2024); https://doi.org/10.1117/12.3012857
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KEYWORDS
Proteins

Bacteria

Double sideband modulation

Design and modelling

Genetics

Genomics

Resistance

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