Competitive and sandwich TIRF immunosensors: a model for performance optimization

Antonio Schirone; Michele S. Celebre; Claudio Domenici; Danilo De Rossi; Arti Ahluwalia; Lucia Mosiello

doi:10.1117/12.144737

18 May 1993 Competitive and sandwich TIRF immunosensors: a model for performance optimization

Antonio Schirone, Michele S. Celebre, Claudio Domenici, Danilo De Rossi, Arti Ahluwalia, Lucia Mosiello

Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144737
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

The effects of some fundamental parameters on the performance of optical immunosensors based on fluorescence induced by an evanescent wave (total internal reflection fluorescence, or TIRF) are evaluated. Both competitive and sandwich systems are discussed and compared. The performance of such devices are described using the range within which the error is less than a fixed value. Methods of controlling the effects of various parameters are considered.

Citation Download Citation

Antonio Schirone, Michele S. Celebre, Claudio Domenici, Danilo De Rossi, Arti Ahluwalia, and Lucia Mosiello "Competitive and sandwich TIRF immunosensors: a model for performance optimization", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144737

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