Liquid chromatography combined with visible diode-laser-induced fluorescence detection for photosensitizers

Arjan J.G. Mank; Cees Gooijer; Henk Lingeman; Nel H. Velthorst; Udo A. T. Brinkman

doi:10.1117/12.167312

1 February 1994 Liquid chromatography combined with visible diode-laser-induced fluorescence detection for photosensitizers

Arjan J.G. Mank, Cees Gooijer, Henk Lingeman, Nel H. Velthorst, Udo A. T. Brinkman

Author Affiliations +

Proceedings Volume 2084, Biomedical Optoelectronic Devices and Systems; (1994) https://doi.org/10.1117/12.167312
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

A combination of conventional-size liquid chromatography and visible diode-laser induced fluorescence (DIO-LIF) detection has been developed and applied for the detection of a model photosensitizer, AlPcS₂, in biological samples. Conventional fluorimetry was found to give unsatisfactory information. A detection limit of 2.1 X 10^-12 M (S/N equals 3) was obtained for AlPcS₂ in both rat urine and faeces extracts using the DIO-LIF system, 20-fold lower than obtained with a conventional fluorescence detection system, when used in combination with chromatography.

Citation Download Citation

Arjan J.G. Mank, Cees Gooijer, Henk Lingeman, Nel H. Velthorst, and Udo A. T. Brinkman "Liquid chromatography combined with visible diode-laser-induced fluorescence detection for photosensitizers", Proc. SPIE 2084, Biomedical Optoelectronic Devices and Systems, (1 February 1994); https://doi.org/10.1117/12.167312

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available