Registration of damages of endothelial cell cultures by beta-nicotinamide adenine dinucleotide (NADH) fluorescence

Werner Schramm; Mathias Nittka; Wolfgang Hoehne; Heinz Detlef Kronfeldt; Joerg Rauschenberg

doi:10.1117/12.200883

1 February 1995 Registration of damages of endothelial cell cultures by beta-nicotinamide adenine dinucleotide (NADH) fluorescence

Werner Schramm, Mathias Nittka, Wolfgang Hoehne, Heinz Detlef Kronfeldt, Joerg Rauschenberg

Author Affiliations +

Proceedings Volume 2329, Optical and Imaging Techniques in Biomedicine; (1995) https://doi.org/10.1117/12.200883
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

NADH is an indispensable mediator of energy metabolism in cells. By laser fluorescence spectroscopy with an experimental setup we studied the influence of different concentrations of NaCN solutions on the NADH fluorescence intensity of endothelial cell cultures of the calf aorta (BKz-7). The results obtained are discussed against the background of published cytotoxicity studies of cyanides with endothelial cell cultures.

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Werner Schramm, Mathias Nittka, Wolfgang Hoehne, Heinz Detlef Kronfeldt, and Joerg Rauschenberg "Registration of damages of endothelial cell cultures by beta-nicotinamide adenine dinucleotide (NADH) fluorescence", Proc. SPIE 2329, Optical and Imaging Techniques in Biomedicine, (1 February 1995); https://doi.org/10.1117/12.200883

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