Raman scattering spectra of insect virus PrGV-VP and their surface-enhanced Raman scattering spectra adsorbed on silver

PeiDi Bao; TianQuan Huang; XinMing Liu; Tieqiao Wu

doi:10.1117/12.305384

16 April 1998 Raman scattering spectra of insect virus PrGV-VP and their surface-enhanced Raman scattering spectra adsorbed on silver

PeiDi Bao, TianQuan Huang, XinMing Liu, Tieqiao Wu

Author Affiliations +

Proceedings Volume 3250, Optical Biopsy II; (1998) https://doi.org/10.1117/12.305384
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

The ordinary Raman scattering (ORS) spectra of virion protein of Granulosis Virus of Cabbage Butterfly Preris rapae (PrGV-VP) and their surface-enhanced Raman scattering (SERS) spectra adsorbed on the surface of silver colloid particles have been found that the Raman signal in the SERS spectra was enhanced by a factor of about 8 X 10². In ORS spectra of PrGV-VP, it indicates that PrGV-VP has a predominantly random-coil secondary structures. All tyrosine residues are exposed. And the PrGV-VP has a gauche-gauche- gauche configuration of the C-C-S-S-C-C linkage. In SERS spectra of PrGV-VP, the intensities of bands related to COO group and NH₂ group vibrations are relatively strong. We interpret that the PrGV-VP are adsorbed on the Ag surface through both the carboxylate groups and the amino groups. The efficient enhancement of amide I and amide II vibrations are impeded, because the peptide groups of PrGV-VP are screened by the side-chains amino acid residues. The Raman enhancement of PrGV-VP has a short-range mechanism. The chemisorption is a main mechanism on Ag hydrosols of PrGV- VP.

Citation Download Citation

PeiDi Bao, TianQuan Huang, XinMing Liu, and Tieqiao Wu "Raman scattering spectra of insect virus PrGV-VP and their surface-enhanced Raman scattering spectra adsorbed on silver", Proc. SPIE 3250, Optical Biopsy II, (16 April 1998); https://doi.org/10.1117/12.305384

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