Multiwavelength spectroscopy for the detection, identification, and quantification cells

Yvette D. Mattley; Luis H. Garcia-Rubio

doi:10.1117/12.418743

13 March 2001 Multiwavelength spectroscopy for the detection, identification, and quantification cells

Yvette D. Mattley, Luis H. Garcia-Rubio

Proceedings Volume 4206, Photonic Detection and Intervention Technologies for Safe Food; (2001) https://doi.org/10.1117/12.418743
Event: Environmental and Industrial Sensing, 2000, Boston, MA, United States

Abstract

Multiwavelength spectroscopy is a rapid technique that provides quantitative information for the detection and identification of cells. A typical multiwavelength spectrum reflects the chemical composition, size, internal structure arid number of cells present in a sample. These properties constitute essential information for the identification and classification of cells. The multiwavelength spectrum is generated from the combined scattering and absorption characteristics of the sample. Light scattering theory is then used to deconvolute the spectrum for estimates ofthe critical parameters necessary for the detection and identification of cells. This approach has been used to determine the spectral fingerprint for blood cells, bacterial cells and protozoa. The characteristic set of optical properties for platelets, E. coil and Cryptosporidium have been determined as a function of wavelength and used for the quantitative interpretation ofUV-vis spectra within the context ofMie theory. The models developed using this approach provide reliable and accurate estimates for cell size, number, chemical composition and internal structure. Information on the chemical composition is further deconvoluted into quantitative estimates of nucleic acid and protein content. This type of detailed information is thell used for the discrimination of cell types. The technique is applicable to a wide range of cell types found in diverse environments. Advances in the development of miniaturized spectrometers increase the potential of this method as an excellent candidate for a rapid, reliable and efficient biosensor.

Citation Download Citation

Yvette D. Mattley and Luis H. Garcia-Rubio "Multiwavelength spectroscopy for the detection, identification, and quantification cells", Proc. SPIE 4206, Photonic Detection and Intervention Technologies for Safe Food, (13 March 2001); https://doi.org/10.1117/12.418743

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