Detection of DNA hybridization with surface plasmon resonance biosensor: comparison of immobilization of oligonucleotides by ssDNA and dsDNA

Meng Wu; Zhihong Lin; Vladimir Mirsky; Otto S. Wolfbeis

doi:10.1117/12.440180

14 September 2001 Detection of DNA hybridization with surface plasmon resonance biosensor: comparison of immobilization of oligonucleotides by ssDNA and dsDNA

Meng Wu, Zhihong Lin, Vladimir Mirsky, Otto S. Wolfbeis

Author Affiliations +

Proceedings Volume 4414, International Conference on Sensor Technology (ISTC 2001); (2001) https://doi.org/10.1117/12.440180
Event: International Conference on Sensing units and Sensor Technology, 2001, Wuhan, China

Abstract

In this research, a surface-plasmon resonance biosensor for nucleic acid detection was constructed, optimized and characterized. The kinetic response on hybridization was also studied. The biosensor was use as a platform for the study of the surface coverage control by single-strand or double-strand oligonucleotide immobilization. The dsDNA immobilization and offers a new way to surface coverage control for the nucleic acid biosensor construction.

Citation Download Citation

Meng Wu, Zhihong Lin, Vladimir Mirsky, and Otto S. Wolfbeis "Detection of DNA hybridization with surface plasmon resonance biosensor: comparison of immobilization of oligonucleotides by ssDNA and dsDNA", Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); https://doi.org/10.1117/12.440180

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