New method for monitoring nitric oxide in vivo using microdialysis sampling and chemiluminescence reaction

Dachun Yao; Nick P. Evmiridis; Yikai Zhou; Shunqing Xu; Huarong Zhou

doi:10.1117/12.440157

14 September 2001 New method for monitoring nitric oxide in vivo using microdialysis sampling and chemiluminescence reaction

Dachun Yao, Nick P. Evmiridis, Yikai Zhou, Shunqing Xu, Huarong Zhou

Author Affiliations +

Proceedings Volume 4414, International Conference on Sensor Technology (ISTC 2001); (2001) https://doi.org/10.1117/12.440157
Event: International Conference on Sensing units and Sensor Technology, 2001, Wuhan, China

Abstract

A new method employing a combination of micro dialysis sampling and chemiluminescence reaction was developed to monitor nitric oxide (NO) in vivo. A special probe was designed with an interference-free membrane to achieve a very high selectivity for NO. High sensitivity was achieved by optimizing the working system and improving the NO sampling time. This system was used in vivo to monitor blood and brain tissue in rats and rabbits. We have established that this system is sensitive enough to detect variations in NO production in difference physiological state. The system can detect NO in the linear range of 5nM-1(mu) M, with a detection limit of 1nM, and real NO concentrations in our experimental animals were found to be in the range of 1-5 nM or even less. Finally, the effects of body temperature, NO donors, Viagra, NO activators, NO cofactors, NO interference were investigated carefully in different physiological situations.

Citation Download Citation

Dachun Yao, Nick P. Evmiridis, Yikai Zhou, Shunqing Xu, and Huarong Zhou "New method for monitoring nitric oxide in vivo using microdialysis sampling and chemiluminescence reaction", Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); https://doi.org/10.1117/12.440157

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available