Cell adhesion and guidance by micropost-array chemical sensors

Paul Pantano; Soo-Kim Quah; Kristine L. Danowski

doi:10.1117/12.469778

4 June 2002 Cell adhesion and guidance by micropost-array chemical sensors

Paul Pantano, Soo-Kim Quah, Kristine L. Danowski

Proceedings Volume 4625, Clinical Diagnostic Systems: Technologies and Instrumentation; (2002) https://doi.org/10.1117/12.469778
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

An array of ~50,000 individual polymeric micropost sensors was patterned across a glass coverslip by a photoimprint lithographic technique. Individual micropost sensors were ~3-micrometers tall and ~8-micrometers wide. The O₂-sensitive micropost array sensors (MPASs) comprised a ruthenium complex encapsulated in a gas permeable photopolymerizable siloxane. The pH-sensitive MPASs comprised a fluorescein conjugate encapsulated in a photocrosslinkable poly(vinyl alcohol)-based polymer. PO₂ and pH were quantitated by acquiring MPAS luminescence images with an epifluorescence microscope/charge coupled device imaging system. O₂-sensitive MPASs displayed linear Stern-Volmer quenching behavior with a maximum I_o/I of ~8.6. pH-sensitive MPASs displayed sigmoidal calibration curves with a pK_a of ~5.8. The adhesion of undifferentiated rat pheochromocytoma (PC12) cells across these two polymeric surface types was investigated. The greatest PC12 cell proliferation and adhesion occurred across the poly(vinyl alcohol)-based micropost arrays relative to planar poly(vinyl alcohol)-based surfaces and both patterned and planar siloxane surfaces. An additional advantage of the patterned MPAS layers relative to planar sensing layers was the ability to direct the growth of biological cells. Preliminary data is presented whereby nerve growth factor-differentiated PC12 cells grew neurite-like processes that extended along paths defined by the micropost architecture.

Citation Download Citation

Paul Pantano, Soo-Kim Quah, and Kristine L. Danowski "Cell adhesion and guidance by micropost-array chemical sensors", Proc. SPIE 4625, Clinical Diagnostic Systems: Technologies and Instrumentation, (4 June 2002); https://doi.org/10.1117/12.469778

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