Integrated micro-/nanofabrication of cell culture scaffolds with selective cell adhesion and fluorescent indicators

Javeed Shaikh-Mohammed; Mengyan Li; Deepti Terala; Michael J. McShane

doi:10.1117/12.524644

23 December 2003 Integrated micro-/nanofabrication of cell culture scaffolds with selective cell adhesion and fluorescent indicators

Javeed Shaikh-Mohammed, Mengyan Li, Deepti Terala, Michael J. McShane

Author Affiliations +

Proceedings Volume 5345, Microfluidics, BioMEMS, and Medical Microsystems II; (2003) https://doi.org/10.1117/12.524644
Event: Micromachining and Microfabrication, 2004, San Jose, California, United States

Abstract

This work reports the micro- and nano-fabrication of substrates obtained by coupling conventional photolithography and layer-by-layer (LbL) self-assembly technologies, and the application for building in vitro cell culture scaffolds. Using photolithography, cell culture scaffolds of various shapes (circles, squares, and rectangles) and sizes (10 to 300 μm) were obtained using direct and soft lithography methods in SU-8, polydimethylsiloxane (PDMS), and Poly(methyl methacrylate) (PMMA). Using LbL technology, patterned polyelectrolyte ultrathin multilayer films, containing gelatin to promote cell adhesion, were then deposited on or between raised microstructures, depending on the intended application of the cell culture scaffolds. Fluorescent indicator dyes are integrated onto the microstructures to allow monitoring changes of pH and oxygen in the cell culture media. Thus, cell activity may be monitored by using the fluorescent film properties, enabling a high-throughput cell-based in vitro screening system. The findings indicate that combining conventional photolithography and LbL technologies is an efficient method to fabricate micro- and nanoscale patterns as substrates to modulate the surface properties of in vitro cell culture scaffolds.

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Javeed Shaikh-Mohammed, Mengyan Li, Deepti Terala, and Michael J. McShane "Integrated micro-/nanofabrication of cell culture scaffolds with selective cell adhesion and fluorescent indicators", Proc. SPIE 5345, Microfluidics, BioMEMS, and Medical Microsystems II, (23 December 2003); https://doi.org/10.1117/12.524644

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KEYWORDS

Optical lithography

Glasses

Polymethylmethacrylate

Lithography

Thin films

Fabrication

In vitro testing

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