PCR device with integrated thermal cycling and fluorescence detection elements

M. Bahrami; T. Melvin; J. S. Wilkinson; A. G. R. Evans

doi:10.1117/12.608646

1 July 2005 PCR device with integrated thermal cycling and fluorescence detection elements

M. Bahrami, T. Melvin, J. S. Wilkinson, A. G. R. Evans

Proceedings Volume 5836, Smart Sensors, Actuators, and MEMS II; (2005) https://doi.org/10.1117/12.608646
Event: Microtechnologies for the New Millennium 2005, 2005, Sevilla, Spain

Abstract

In this paper, we present both a theoretical evaluation and the fabrication of a novel polymerase chain reaction (PCR) microdevice. This microdevice contains elements for both thermal cycling and fluorescence detection. The proposed device is composed of a reaction chamber with integrated temperature sensor, heaters, p-n diode and optical filter. The advantage of combining these in a single structure is that real time detection of DNA amplification will be possible using a small volume of the PCR solution. The photodiode is covered by a thin film optical filter in order to block out the light which is used to excite the fluorophore dyes. CdS is used for the first time for such a filter and the complete micro-fabrication process is described.

Citation Download Citation

M. Bahrami, T. Melvin, J. S. Wilkinson, and A. G. R. Evans "PCR device with integrated thermal cycling and fluorescence detection elements", Proc. SPIE 5836, Smart Sensors, Actuators, and MEMS II, (1 July 2005); https://doi.org/10.1117/12.608646

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