Singlet oxygen kinetics inside living cells: observation of endogenous quencher consumption and consequences for the spatial resolution in time-resolved measurements

Steffen Hackbarth; Jan Schlothauer; Annegret Preuß; Beate Röder

doi:10.1117/12.822830

13 July 2009 Singlet oxygen kinetics inside living cells: observation of endogenous quencher consumption and consequences for the spatial resolution in time-resolved measurements

Steffen Hackbarth, Jan Schlothauer, Annegret Preuß, Beate Röder

Author Affiliations +

Proceedings Volume 7380, Photodynamic Therapy: Back to the Future; 738045 (2009) https://doi.org/10.1117/12.822830
Event: 12th World Congress of the International Photodynamic Association, 2009, Seattle, Washington, United States

Abstract

The consumption of singlet oxygen quenchers during illumination of cells incubated with photosensitizers is a serious issue for the determination of singlet oxygen kinetics in cells. Using LNCaP cells incubated with pheophorbide a it will be shown, that already an illumination of 100 nJ/cell dramatically changes the value of the observables. This finding has consequences for the spatial resolution of any time-resolved singlet oxygen measurement, since the radiative rate constant of singlet oxygen in aqueous solutions is very small.

Citation Download Citation

Steffen Hackbarth, Jan Schlothauer, Annegret Preuß, and Beate Röder "Singlet oxygen kinetics inside living cells: observation of endogenous quencher consumption and consequences for the spatial resolution in time-resolved measurements", Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 738045 (13 July 2009); https://doi.org/10.1117/12.822830

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