Combination of angiogenesis inhibitors increases the anti-tumor efficacy of photodynamic therapy in a human bladder tumor xenograft model

Ramaswamy Bhuvaneswari; Yik Yuen Gan; Patricia S. P. Thong; William Wei Lim Chin; Khee Chee Soo; Malini Olivo

doi:10.1117/12.828460

13 July 2009 Combination of angiogenesis inhibitors increases the anti-tumor efficacy of photodynamic therapy in a human bladder tumor xenograft model

Ramaswamy Bhuvaneswari, Yik Yuen Gan, Patricia S. P. Thong, William Wei Lim Chin, Khee Chee Soo, Malini Olivo

Author Affiliations +

Proceedings Volume 7380, Photodynamic Therapy: Back to the Future; 73806D (2009) https://doi.org/10.1117/12.828460
Event: 12th World Congress of the International Photodynamic Association, 2009, Seattle, Washington, United States

Abstract

Photodynamic therapy (PDT) is a standard treatment for various malignant and non-malignant conditions. Though therapeutic responses are encouraging, recurrences have been noted, as one of the limitations of PDT is treatment-induced hypoxia that triggers angiogenesis. The present study evaluates the use of angiogenic inhibitors Avastin, that targets vascular endothelial growth factor (VEGF) and Erbitux that targets epidermal growth factor receptor (EGFR) with PDT in an in vivo bladder carcinoma xenograft. Tumor bearing mice were assigned to 6 different categories: control, PDT only, Avastin + Erbitux, PDT + Avastin, PDT + Erbitux and PDT + Avastin and Erbitux. Treated and control tumors were monitored for recurrence for up to 90 days. VEGF and EGFR expression was detected in the tumor tissue. Migratory assay was performed to establish the inhibitory effect of the angiogenesis agents. Using confocal laser endomicroscopy, the tumor microvasculature was assessed. Tumors treated with the combination therapy of PDT + inhibitors showed significantly greater response compared to control and PDT only treated group. Combination therapy treated tumors also showed the most post-treatment damage with reduced tumor vasculature. These results demonstrate that the combination of PDT with inhibitors that target different angiogenesis pathways can improve tumor control.

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Ramaswamy Bhuvaneswari, Yik Yuen Gan, Patricia S. P. Thong, William Wei Lim Chin, Khee Chee Soo, and Malini Olivo "Combination of angiogenesis inhibitors increases the anti-tumor efficacy of photodynamic therapy in a human bladder tumor xenograft model", Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 73806D (13 July 2009); https://doi.org/10.1117/12.828460

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KEYWORDS

Tumors

Photodynamic therapy

Bladder

Blood vessels

Confocal microscopy

In vivo imaging

Endomicroscopy

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