Dynamic complex optical fields for optical manipulation, 3D microscopy, and photostimulation of neurotransmitters

Vincent Ricardo Daria; Christian Stricker; John Bekkers; Steve Redman; Hans Bachor

doi:10.1117/12.862388

27 August 2010 Dynamic complex optical fields for optical manipulation, 3D microscopy, and photostimulation of neurotransmitters

Vincent Ricardo Daria, Christian Stricker, John Bekkers, Steve Redman, Hans Bachor

Author Affiliations +

Proceedings Volume 7762, Optical Trapping and Optical Micromanipulation VII; 77621S (2010) https://doi.org/10.1117/12.862388
Event: SPIE NanoScience + Engineering, 2010, San Diego, California, United States

Abstract

We demonstrate a multi-functional system capable of multiple-site two-photon excitation of photo-sensitive compounds as well as transfer of optical mechanical properties on an array of mesoscopic particles. We use holographic projection of a single Ti:Sapphire laser operating in femtosecond pulse mode to show that the projected three-dimensional light patterns have sufficient spatiotemporal photon density for multi-site two-photon excitation of biological fluorescent markers and caged neurotransmitters. Using the same laser operating in continuous-wave mode, we can use the same light patterns for non-invasive transfer of both linear and orbital angular momentum on a variety of mesoscopic particles. The system also incorporates high-speed scanning using acousto-optic modulators to rapidly render 3D images of neuron samples via two-photon microscopy.

Citation Download Citation

Vincent Ricardo Daria, Christian Stricker, John Bekkers, Steve Redman, and Hans Bachor "Dynamic complex optical fields for optical manipulation, 3D microscopy, and photostimulation of neurotransmitters", Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 77621S (27 August 2010); https://doi.org/10.1117/12.862388

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