Fluorescence intensity fluctuations in single-molecule polarization sensitive measurements

Lior Turgeman; Dror Fixler

doi:10.1117/12.907270

13 February 2012 Fluorescence intensity fluctuations in single-molecule polarization sensitive measurements

Lior Turgeman, Dror Fixler

Proceedings Volume 8228, Single Molecule Spectroscopy and Superresolution Imaging V; 82280I (2012) https://doi.org/10.1117/12.907270
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

A general framework to include fluctuations in the single molecule fluorescence intensity (FI) signal due to random changes in molecule dipole orientation was introduced at Optics Express (21, 2007). By assuming continuous changes in dipole orientation described by Brownian rotational diffusion, this research derives the probability density function (PDF) equation of FI fluctuations. Solution of the proposed equation for several limiting cases and different correlation times yields the short time behavior of FI fluctuations. Monte Carlo simulations results, in accordance with those found in theory will be presented during our talk.

Citation Download Citation

Lior Turgeman and Dror Fixler "Fluorescence intensity fluctuations in single-molecule polarization sensitive measurements", Proc. SPIE 8228, Single Molecule Spectroscopy and Superresolution Imaging V, 82280I (13 February 2012); https://doi.org/10.1117/12.907270

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